Role of tyrosine 129 in the active site of spinach glycolate oxidase

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66136/1/j.1432-1033.1993.tb17852.x.pd

The role of the novel D2/β2-agonist, Viozan™ (sibenadet HCl), in the treatment of symptoms of chronic obstructive pulmonary disease: results of a large-scale clinical investigation

AbstractViozan™ (sibenadet HCl, AR-C68397AA) is a novel dual D2 dopamine receptor, β2-adrenoceptor agonist, developed specifically to treat the key symptoms of chronic obstructive pulmonary disease (COPD), breathlessness, cough and sputum. The dual sensory nerve modulation and bronchodilator effects of sibenadet have been demonstrated in initial dose-ranging studies of patients with COPD and large-scale clinical evaluation has now been completed. Sibenadet efficacy was determined by assessing symptomatic changes, as defined by the novel assessment tool, the Breathlessness, Cough and Sputum Scale (BCSS©). The findings of two placebo-controlled studies are reported.These multicentre, double-blind, placebo-controlled studies recruited over 2000 patients with stable COPD, randomized to receive sibenadet (500 μg) or placebo, pressurized metered-dose inhaler (pMDI) (three times daily) for a period of 12 or 26 weeks. Diary cards were completed daily by patients throughout the study to record BCSS scores, peak expiratory flow (PEF), study drug and rescue bronchodilator usage, changes in concomitant medication and adverse events. The primary endpoints were defined as change from baseline to the final 4 weeks of the treatment period in mean BCSS total score, and forced expiratory volume in one second (FEV1) measured 1 hour after administration of the final dose of study drug and expressed as a percentage of the predicted FEV1. In addition, clinic assessments were made to determine changes in pulmonary function, health-related quality of life, perception of treatment efficacy and adverse events.Despite initial improvements in mean daily BCSS total scores in patients receiving sibenadet, the difference in the change from baseline to the final 4 weeks of the treatment period between the two treatment groups was neither statistically significant, nor considered to be of clinical importance. Although marked bronchodilator activity was seen early on with sibenadet treatment, the duration of effect diminished as the studies progressed. Sibenadet use was not associated with any safety concerns.These studies, utilizing the novel BCSS, have clearly illustrated that, despite initial symptomatic improvement with sibenadet therapy, this clinical benefit was not sustained over the course of the study

Imaging the circumstellar envelopes of AGB stars

We report the results of an exploratory program to image the extended circumstellar envelopes of asymptotic giant branch (AGB) stars in dust-scattered galactic light. The goal is to characterize the morphology of the envelopes as a probe of the mass-loss process. The observations consist of short exposures with the VLT and longer exposures with 1-2m telescopes, augmented with archival images from the Hubble Space Telescope. We observed 12 AGB stars and detected the circumstellar envelopes in 7. The detected envelopes have mass loss rates more than about 5 10E-6 solar mass per year, and they can be seen out to distances of about 1 kpc. The observations provide information on the mass loss history on time scales up to about 10,000 years. For the five AGB envelopes in which the circumstellar geometry is well determined by scattered light observations, all except one (OH348.2-19.7) show deviations from spherical symmetry. Two (IRC+10216 and IRC+10011) show roughly spherical envelopes at large radii but asymmetry or bipolarity close to the star; one (AFGL 2514) shows an extended, elliptical envelope, and one (AFGL 3068) shows a spiral pattern. The non-spherical structures are all consistent with the effects of binary interactions. Our observations are in accord with a scenario in which binary companions play a role in shaping planetary nebulae, and show that the circumstellar gas is already partly shaped on the AGB, before evolution to the proto-planetary nebula phase.Comment: Accepted by AA 21 Feb 2006; 18 pages, 14 figs; for high resolution images, contact mauron at graal.univ-montp2.f

A Detailed Analysis of the Dust Formation Zone of IRC+10216 Derived from Mid-IR Bands of C2H2 and HCN

A spectral survey of IRC+10216 has been carried out in the range 11 to 14 um with a spectral resolution of about 4 km s^-1. We have identified a forest of lines in six bands of C2H2 involving the vibrational states from the ground to 3nu5 and in two bands of HCN, involving the vibrational states from the ground up to 2nu2. Some of these transitions are observed also in H13CCH and H13CN. We have estimated the kinetic, vibrational, and rotational temperatures, and the abundances and column densities of C2H2 and HCN between 1 and 300 R* (1.5E16 cm) by fitting about 300 of these ro-vibrational lines. The envelope can be divided into three regions with approximate boundaries at 0.019 arcsec (the stellar photosphere), 0.1 arcsec (the inner dust formation zone), and 0.4 arcsec (outer dust formation zone). Most of the lines might require a large microturbulence broadening. The derived abundances of C2H2 and HCN increase by factors of 10 and 4, respectively, from the innermost envelope outwards. The derived column densities for both C2H2 and HCN are 1.6E19 cm^-2. Vibrational states up to 3000 K above ground are populated, suggesting pumping by near-infrared radiation from the star and innermost envelope. Low rotational levels can be considered under LTE while those with J>20-30 are not thermalized. A few lines require special analysis to deal with effects like overlap with lines of other molecules.Comment: 8 pages, 16 figures, 2 machine-readable tables, accepted in the Astrophysical Journa

Point Sources from a Spitzer IRAC Survey of the Galactic Center

We have obtained Spitzer/IRAC observations of the central 2.0 x 1.4 degrees (~280 x 200 pc) of the Galaxy at 3.6-8.0 microns. A point source catalog of 1,065,565 objects is presented. The catalog includes magnitudes for the point sources at 3.6, 4.5, 5.8, and 8.0 microns, as well as JHK photometry from 2MASS. The point source catalog is confusion limited with average limits of 12.4, 12.1, 11.7, and 11.2 magnitudes for [3.6], [4.5], [5.8], and [8.0], respectively. We find that the confusion limits are spatially variable because of stellar surface density, background surface brightness level, and extinction variations across the survey region. The overall distribution of point source density with Galactic latitude and longitude is essentially constant, but structure does appear when sources of different magnitude ranges are selected. Bright stars show a steep decreasing gradient with Galactic latitude, and a slow decreasing gradient with Galactic longitude, with a peak at the position of the Galactic center. From IRAC color-magnitude and color-color diagrams, we conclude that most of the point sources in our catalog have IRAC magnitudes and colors characteristic of red giant and AGB stars.Comment: 44 pages, 13 figures, ApJS in pres

Hyperstrong Radio-Wave Scattering in the Galactic Center. II. A Likelihood Analysis of Free Electrons in the Galactic Center

The scattering diameters of Sgr A* and several nearby OH masers (~ 1" at 1 GHz) indicate that a region of enhanced scattering is along the line of sight to the Galactic center. We combine radio-wave scattering data and free-free emission and absorption measurements in a likelihood analysis that constrains the following parameters of the GC scattering region: The GC-scattering region separation, d; the angular extent of the region, \psi_l; the outer scale on which density fluctuations occur, l_0; and the gas temperature, T. The maximum likelihood estimates of these parameters are d = 133_{-80}^{+200} pc, 0.5 degrees <= \psi_l <~ 1 degrees, and (l_0/1 pc)^{2/3}T^{-1/2} = 10^{-7 +/- 0.8}. As host media for the scattering, we consider the photoionized surface layers of molecular clouds and the interfaces between molecular clouds and the 10^7 K ambient gas. We are unable to make an unambiguous determination, but we favor an interface model in which the scattering medium is hot (T ~ 10^6 K) and dense (n_e ~ 10 cm^{-3}). The GC scattering region produces a 1 GHz scattering diameter for an extragalactic source of 90", if the region is a single screen, or 180", if the region wraps around the GC, as appears probable. We modify the Taylor-Cordes model for the Galactic distribution of free electrons in order to include an explicit GC component. Pulsars seen through this region will have a dispersion measure of approximately 2000 pc cm^{-3}, of which 75% arises from the GC component. We stress the uniqueness of the GC scattering region, probably resulting from the high-pressure environment in the GC.Comment: 39 pages with 9 PostScript figures; LaTeX2e with AASTeX macro aaspp4, to be published in Ap

The wonderful complexity of the Mira AB system

We have mapped the CO(3-2) line emission around the Mira AB system at 0.5 resolution using the Atacama Large Millimeter/submillimeter Array (ALMA). The CO map shows amazing complexity. The circumstellar gas has been shaped by different dynamical actors during the evolution of the system and several morphological components can be identified. The companion is marginally resolved in continuum emission and is currently at 0.487$\pm$0.006 separation. In the main line component, centered on the stellar velocity, spiral arcs around Mira A are found. The spiral appears to be relatively flat and oriented in the orbital plane. An accretion wake behind the companion is clearly visible and the projected arc separation is of order 5''. In the blue wing of the line emission, offset from the main line, several large ($\sim$5-10''), opposing arcs are found. We tentatively suggest that this structure is created by the wind of Mira B blowing a bubble in the expanding envelope of Mira A.Comment: Letter accepted in A&

Stellar Proper Motions in the Galactic Bulge from deep HST ACS/WFC Photometry

We present stellar proper motions in the Galactic bulge from the Sagittarius Window Eclipsing Extrasolar Search (SWEEPS) project using ACS/WFC on HST. Proper motions are extracted for more than 180,000 objects, with >81,000 measured to accuracy better than 0.3 mas/yr in both coordinates. We report several results based on these measurements: 1. Kinematic separation of bulge from disk allows a sample of >15,000 bulge objects to be extracted based on >6-sigma detections of proper motion, with <0.2% contamination from the disk. This includes the first detection of a candidate bulge Blue Straggler population. 2. Armed with a photometric distance modulus on a star by star basis, and using the large number of stars with high-quality proper motion measurements to overcome intrinsic scatter, we dissect the kinematic properties of the bulge as a function of distance along the line of sight. This allows us to extract the stellar circular speed curve from proper motions alone, which we compare with the circular speed curve obtained from radial velocities. 3. We trace the variation of the {l,b} velocity ellipse as a function of depth. 4. Finally, we use the density-weighted {l,b} proper motion ellipse produced from the tracer stars to assess the kinematic membership of the sixteen transiting planet candidates discovered in the Sagittarius Window; the kinematic distribution of the planet candidates is consistent with that of the disk and bulge stellar populations.Comment: 71 pages, 30 figures, ApJ Accepte